YocumRidge Posted October 22, 2009 Posted October 22, 2009 Today’s Feature Article at work: "Mental retardation affects a larger number of males in the population than females, and this has been attributed, in part, to the increased proportion of mutations in genes on the X chromosome. The number of causative mutations leading to X-linked mental retardation (XLMR) is unknown, and will remain so until all of the genes on the X chromosome have been fully investigated. While this research is ongoing, screening for the XLMR remains a challenge. Here we describe the direct detection of non syndromic X-linked Mental Retardation (MRX) variants using the Applied Biosystems 3500xL Genetic Analyzer". Quote
olyclimber Posted October 22, 2009 Posted October 22, 2009 i knew there was a reason for my excessive sialorrhea Quote
billcoe Posted October 22, 2009 Posted October 22, 2009 This you Jake? LOL! [video:youtube]http://www.youtube.com/watch?v=1nYx8iX9oX8&feature=popular Quote
Ponderosa Posted October 22, 2009 Posted October 22, 2009 Today’s Feature Article at work: "Mental retardation affects a larger number of males in the population than females, and this has been attributed, in part, to the increased proportion of mutations in genes on the X chromosome. The number of causative mutations leading to X-linked mental retardation (XLMR) is unknown, and will remain so until all of the genes on the X chromosome have been fully investigated. While this research is ongoing, screening for the XLMR remains a challenge. Here we describe the direct detection of non syndromic X-linked Mental Retardation (MRX) variants using the Applied Biosystems 3500xL Genetic Analyzer". The report could have simply stated, "because they have penises." Do I remember correctly/accurately that all human embryos begin essentially female, but males are derived by hormone introduction? Quote
chirp Posted October 22, 2009 Posted October 22, 2009 People on weed don't act that way! Smoke more weed! Quote
tvashtarkatena Posted October 22, 2009 Posted October 22, 2009 that's the dummist artical I ever red. Quote
KaskadskyjKozak Posted October 22, 2009 Posted October 22, 2009 Can we knock it off with the Kevboner threads already? Quote
Off_White Posted October 22, 2009 Posted October 22, 2009 Hey Bill, I think that guy in the surveillance video was faking, this guy on the other hand is the real deal. [video:youtube]http://www.youtube.com/watch?v=UDF1K-wZlnc Quote
billcoe Posted October 22, 2009 Posted October 22, 2009 LOL! Very similar, I remember dropping my military ID just as I was entering the post (walking) after dropping some window pane and taking about 5 min to get it picked up.....quite disconcerting then, funny now. Quote
olyclimber Posted October 22, 2009 Posted October 22, 2009 acid time warp will get you every time Quote
kevbone Posted October 22, 2009 Posted October 22, 2009 Can we knock it off with the Kevboner threads already? Quote
TamaraSlade Posted October 26, 2009 Posted October 26, 2009 Today’s Feature Article at work: "Mental retardation affects a larger number of males ......" [video:youtube] Quote
layton Posted October 27, 2009 Posted October 27, 2009 hey mito, how much big dye you using? I'm trying to reduce our consumption Quote
cory.nyc Posted October 27, 2009 Posted October 27, 2009 its always funny to see someone get out the video camera at someone else's expense. What did we do for entertainment before youtube came out? Quote
YocumRidge Posted October 27, 2009 Author Posted October 27, 2009 hey mito, how much big dye you using? I'm trying to reduce our consumption Using a BigDye Terminator v3.1, we were able to get a decent signal with as low as 1/10 of what is suggested by ABI, i.e. 0.4 ul in the total reaction volume of 10 ul. With a little bit of tweaking, I would say - 1/20, BUT the ratio of your primer and DNA template should be just right, the PCR product uber clean, etc. You do the math: your time versus $$$. We have also switched to formamide as an injection carrier. Quote
olyclimber Posted October 27, 2009 Posted October 27, 2009 i switched to formaldehyde. its cheaper in bulk than Boones or Olde English. Quote
YocumRidge Posted October 27, 2009 Author Posted October 27, 2009 i switched to formaldehyde. its cheaper in bulk than Boones or Olde English. Methanol is cheaper Porter but you might suffer some. Quote
layton Posted October 27, 2009 Posted October 27, 2009 yeah, we use formamide too. we i came in we were using 5uL per 13uL total vol! Do you use the bdt buffer or just toss it (or make your own) Quote
YocumRidge Posted October 27, 2009 Author Posted October 27, 2009 Do you use the bdt buffer or just toss it (or make your own) We used to make our own 10x BDT: 400 mM Tris-HCl, pH = 9.0 10 mM MgCl2 Now, we are using the DNA Sequencing Dilution Buffer III from TekNova (cat. # D1301), super cheap - $40.00 for 500 ml. Quote
olyclimber Posted October 27, 2009 Posted October 27, 2009 being mentally handicapped does mean you get the best parking when going to the store though. so....point for the males. Quote
ivan Posted October 27, 2009 Posted October 27, 2009 uh, did ya'll not see the "no smart talking in fucking spray" sign when ya came in? jayb no doubt started us down this here slippery slope there, i said slope, so we can have a racial conversation now instead Quote
Recommended Posts
Join the conversation
You can post now and register later. If you have an account, sign in now to post with your account.